Transgenic Mouse Core Facility Overview
Mission
The Transgenic Mouse Core Facility provides investigators at Wake Forest University Health Sciences (WFUHS) access to state-of-the-art technologies for the generation of genetically-engineered mouse lines.
Services
- Consultation with the Core Director regarding the strategy for production and testing of the transgenic and gene targeting constructs
- Pronuclear microinjection of DNA into fertilized mouse embryos for the production of transgenic mouse lines
- Gene targeting in mouse embryonic stem (ES) cells
- Injection of gene-targeted ES cells into mouse blastocysts to produce chimeric mice for the creation of Knock-out/Knock-in mouse lines
- Embryo rederivation for eradication of horizontally-transmitted, post-implantation, and vertically transmitted pathogens
- Mouse genotyping from tail clips, using polymerase chain reaction (PCR) procedures
Details
Staff
Additional Information:
These Cell Migration Consortium/Nature Publishing Group websites provide useful background or resource material for those unfamiliar with the procedures:
- Beginners with Transgenic or Knockout Mice, start here.
- For a guide to the basics of preparing Gene Targeting Constructs, go here.
Note: If you cannot make your own gene targeting constructs, there are companies that will make gene targeting constructs for a fee. The Transgenic Mouse Core staff have not had direct experience with any of these companies, but would be happy to provide investigators with their names. Call Dr. Liqing Yu at 716-0920 for more information.
ES Cell Lines Available for Generating Some Knockout Mice
Some knockout mice can be made without the investigator having to generate the targeting constructs or the targeted mouse embryonic cells. Please check the following links to see if the gene of interest has been targeted in the mouse embryonic stem cells or even in mice.
The International Mouse Knockout Consortium:
Other Useful Weblinks:
Call the Transgenic Mouse Core Facility's director, Dr. Liqing Yu, at 716-0920 for more information.
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Internal Advisory Committee