Lipoprotein Analytic Laboratory

Laboratory Purpose:

To provide analytical and quantitative measurements of lipid and lipoprotein endpoints for non-diagnostic investigational purposes.

Capabilities:

• Apolipoprotein AI, AII, B, E, and Lp(a) quantitation by ELISA
• HDL subfraction analysis by GGE
• Lipoprotein fractionation and estimation of LDL particle size by HPLC
• Lipoprotein-cholesterol distribution determination
• Fatty acid analysis by GLC
• Cholesterol and plant sterol quantitation by GLC
• Lipoprotein distribution and quantitation by NMR

Instrumentation:

• SLT Spectra Shell Microplate Reader
  Beckman L5-50 and Beckman L8-70
• Ultracentrifuges and 70.1 Ti Rotors
  HP Model 5890 GLC systems with Autosamplers (2)
• ISCO HPLC Systems (2)
• Pharmacia GE4 Electrophoresis Chambers and Power Supplies (3)
• Zeineh Soft Laser Scanning Densitometer (Biomed Instruments)
• 360 MHZ NMR Spectrophotometer, Analogic Corp.

Contact Information:

Telephone Number: (336) 716-2137

Director: Lawrence Rudel, PhD
Tel: (336) 716-2821
Fax: (336) 716-6279
lrudel@wfubmc.edu

Supervisor: Martha Wilson, PhD
Tel: (336) 716-2137
Fax: (336) 716-6279
mdwilson@wfubmc.edu