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The Cellular Imaging Core (formerly known as MICROMED) is a full service microscopy laboratory supplying equipment and technical support for both electron and confocal microscopy; it comprises a shared resource available throughout the institution for both clinical and research purposes.
The EM equipment includes conventional transmission and scanning electron microscopy (TEM and SEM). The facility also houses a laser scanning confocal microscope (LSCM), digital upright and inverted fluorescence microscopes, time-lapse microscopy, laser capture microdissection, and light amplification fluorescence and luminescence systems for non-invasive imaging in living rodents. The LSCM is capable of multiphoton excitation.
This resource exists in a biomedical research environment and supports numerous investigators whose interests encompass both basic and clinical medical science. The facility is considered a core laboratory under the Comprehensive Cancer Center grant, providing additional support for microscopy to investigators affiliated with the Cancer Center.
Although the orientation is biomedical, the facility supports a wide range of research. Faculty and staff also have extensive experience in non-biomedical zoology, botany, and materials science imaging.
Additionally, the laboratory offers numerous specialized research techniques, user training, consultation, and collaboration. The Cellular Imaging Core is equipped to carry out all phases of sample preparation for electron and confocal microscopy with a staff that includes a technical engineer/manager and two technologists. |
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Capabilities:
- TEM (120 keV)
- SEM (5-30 keV)
- LSCM with multiphoton excitation
- Bright field, DIC, phase contrast , polarizing, fluorescence and video light microscopy
- Immunolabeling for LM, EM, SEM and LSCM
- Autoradiography and enzyme cytochemistry
- Cyromicrotomy, CryoIVEM, and EDX compositional microanalysis
- Image processing and analysis
- Laser capture microdissection
- Single cell microinjection
- Tumor and cell imaging using GFP or luciferase reporters in living rodents
Instrumentation:
- Philips 400 120 keV TEM with high tilt goniometer stage
- Philips 515 SEM with backscatter, cathodoluminescence, and EDAX detectors
- Zeiss LSM510 laser scanning confocal system
- Arcturus PixCell II laser microdissection system
- Zeiss Axioplan 2 and Olympus IX-70 fluorescence microscopes
- Zeiss Axiovert time-lapse video microscopy system for cultured cells
- Reichert Ultracut microtomes and cryoultramicrotomes
- Computer workstations with image processing, analysis, and 3-D reconstruction software
- Photographic darkrooms
- Complete specimen preparation facilities
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