Quadrupole Time-of-Flight Mass Spectrometer

Features:

·     Waters/Micromass Q-TOF ApcI US

·     LockSpray enabled exact mass measurements, < 3 ppm

·     Lower detection limit < 3 fmole

·     Data Directed Analysis

·     Digital Dead Time Correction Technology

·     Interfaced with a Waters Cap LC

·     Peak Parking Implemented.

The Q-TOF was recently purchased with funds from the NIH Shared Instrumentation Program.  Acquisition of the Q-TOF gives investigators access to new, powerful proteomic technology while at the same time facilitating analysis of complex lipids. Q-TOF advantages include precise mass measurement (< 3 ppm), sensitivity (< 3 fmole) coupled with the MS/MS capability of sequencing peptides and analyze post-translational modifications. Protein identification is conveniently performed on proteolytic digests (usually trypsin) that are separated on a capillary HPLC column before analysis. Most of the peptides from a tryptic digest are relatively small, 500 to 3000 Da, and contain a basic amino acid making detection and MS/MS sequencing easier.