Analytical Imaging Core Laboratory
Faculty responsible:
Alan J. Townsend, Ph.D.
Phone: (336) 713-7215
FAX: (336) 716-7671
E-mail: atown@wfubmc.edu
Website: http://www.wfubmc.edu/biochem/faculty/Townsend/
Location of facility:
Hanes Building, Room 4048
Laboratory purpose:
A multi-purpose imaging facility for acquisition and analysis of images generated from 2-D radionuclide tracer samples (Phosphorimager), fluorescent tracer samples (Typhoon Imager) or images acquired by scanner or CCD video camera input (Alpha Innotech gel documentation station).
Laboratory capabilities:
- Imaging for quantitative and qualitative analysis of 2-D radioactive or fluorescent samples such as blots or gels, labeled with 32P, 35S, 14C, 125I, 3H, or with tags for fluorescent or chemiluminescent detection, with 3-5 logs of dynamic range.
- Scanner or video camera-based image acquisition, storage, analysis, and printing for documentation of SDS-PAGE and ethidium bromide-stained DNA and RNA gels.
- Multiparametric flow cytometry and cell sorting.
- Generation of cDNA macroarray blots for expression analysis.
Laboratory services:
A part-time technician is available by appointment to train users in the use of self-serve imaging equipment and software (ext. 6-6347, leave message). Temporary data storage (up to 4 months) is allowed on PC hard drives, with at least monthly backup to tape. Users are notified prior to data purging to free hard drive space.
Instrumentation:
- Molecular Dynamics Phosphorimager 445SI (radioactivity detection only)
- MD Typhoon 8600 scanner (radioactive, fluorescent, or chemiluminescent labels)
- Alpha Innotech IS-2000 imaging workstation for gel documentation and analysis
- UMAX Astra 4000 flatbed scanner (1200 dpi) for 2-D color or grayscale imaging
- 35mm slidemaker for direct color image capture to film (e.g. from PowerPoint)
- Two Dell pentium/windows NT workstations with image manipulation and analysis software and a Macintosh iMac with image analysis software and zip drive
- Becton-Dickinson FACStar Plus flow cytometer/cell sorter
- Genomics Solutions FlexSys robotic array spotter