Name: Amy L. Sloat, Ph.D.

Email address: asloat@wfubmc.edu 

Education:        NIH Postdoctoral Research Fellow, Cancer Biology, WFU Health Sciences, 2007-present

Ph.D. Analytical Chemistry, Wake Forest University, 2007

                      B.S. Chemistry, Biochemistry Conc., UNC-Greensboro 2002

                      A.A.S. Science, Forsyth Tech Community College, 2000      

Advisor/Lab:  Steven A. Akman, M.D.

Current Research: The Akman lab is interested in the effects of ethanol induced DNA adducts on DNA replication.  Ethanol is known to result in the formation of O6- and N2-ethyl deoxyguanosine adducts.  These adducts stall the replication process, which can lead to deletions, mutations, and recombination events.  Our objective is to study the 2-dimensional gel electrophoresis replication patterns of stalled replication forks to assess the types of recombination that may be occurring, and the roles of the Y-family polymerases in avoiding, or promoting the aberrant recombination events. 

Publications:

Sloat, A. L.; Roper, M. G.; Lin, X.; Ferrance, J. P.; Landers, J. P.; Colyer, C. L. Protein Determination by Microchip Capillary Electrophoresis Using an Asymmetric Squarylium Dye: Noncovalent Labeling and Nonequilibrium Measurement of Association Constants. Electrophoresis submitted.

Yan, W.; Sloat, A. L.; Yagi, S.; Nakazumi, H.; Colyer, C. L. Protein Labeling with Red Squarylium Dyes for Analysis by Capillary Electrophoresis with Laser-Induced Fluorescence Detection. Electrophoresis 2006, 27, 1347-1354.

Dai, J.; Sloat, A. L.; Wright, M. W.; Manderville, R., Role of Phenoxyl Radicals in DNA Adduction by Chlorophenol Xenobiotics Following Peroxidase Activity. Chemical Research in Toxicology 2005, 18, 771-779.

Honors and Awards: 

NIH Postdoctoral Training Fellowship, WFUHS Cancer Biology Dept, 2007-present

ACS Women’s Chemist Travel Award, Eli Lilly and American Chemical Society, 2006

1^st Place Poster Recipient, 23^rd Triangle Chromatography Symposium, 2006

Department Chair’s Assistantship, Wake Forest University, 2006-2007

Research Assistantship, Wake Forest University, 2004-2006

Dean’s Fellowship, Wake Forest University, 2002-2004

Felton Marble Scholarship, University of North Carolina at Greensboro, 2001-2002

Hobbies – Other:  When not in the lab, I do what I can to get outdoors.  Be it hiking, camping, gardening, or playing sports, time outside is very important to me.