Poole

Dr. Leslie B. Poole

Professor of Biochemistry, Director of the Ctr for Struct Biol
B.A. (Chemistry & Biology), Wake Forest University, 1980
Ph.D. (Biochemistry), Wake Forest University, 1988
Telephone: (336) 716-6711, FAX: (336) 777-3242
E-mail Dr. Poole

Research Interests

Antioxidant enzymes, cysteine-based peroxidases (peroxiredoxins), flavin-containing disulfide reductases, cysteine sulfenic acids in protein function and cell signaling, alkyl hydroperoxide reductases (more info)

Biographical Information

Lab Members

Selected recent publications:

Poole, L.B., and Nelson, K.J.: Discovering mechanisms of signaling-mediated cysteine oxidation. Curr. Opin. Chem. Biol., doi: 10.1016/j.cbpa.2008.01.021 (2008). PDF of article.

Yamamoto, Y., Ritz, D., Planson, A.-G., Jönsson, T.J., Faulkner, M.J., Boyd, D., Beckwith, J., and Poole, L.B.: Mutant AhpC peroxiredoxins suppress thiol-disulfide redox deficiencies and acquire deglutathionylating activity. Mol. Cell 29: 36-45 (2008). PDF of article.

Salsbury Jr., F.R., Knutson, S.T., Poole, L.B., and Fetrow, J.S.: Functional site profiling and electrostatic analysis of cysteines modifiable to cysteine sulfenic acid. Protein Sci. 18: 299-312 (2008). PDF of article.

Nelson, K.J., Day, A.E., Zeng, B.B., King, S.B., and Poole, L.B.: Isotope-coded, iodoacetamide-based reagent to determine individual cysteine pK_a values by MALDI-TOF mass spectrometry. Anal. Biochem. 375: 187-195 (2008). PDF of article.

Parsonage, D., Karplus, P.A., and Poole, L.B.: Substrate specificity and redox potential of AhpC, a bacterial peroxiredoxin. Proc. Natl. Acad. Sci. USA, doi: 10.1073/pnas.0708308105 (2008). PDF of article.

Poole, L.B., Klomsiri, C., Knaggs, S.A., Furdui, C.M., Nelson, K.J., Thomas, M.J., Fetrow, J.S., Daniel, L.W., and King, S.B.: Fluorescent and affinity-based tools to detect cysteine sulfenic acid formation in proteins. Bioconjugate Chem. 18: 2004-2017 (2007). PDF of article.

Michalek, R.D., Nelson, K.J., Holbrook, B.C., Yi, J.S., Stridiron, D., Daniel, L.W., Fetrow, J.S., King, S.B., Poole, L.B., and Grayson, J.M.: The requirement of reversible cysteine sulfenic acid formation for T cell activation and function. J. Immunol. 179: 6456-6467 (2007). PDF of article.

Poole, L.B., King, S.B. and Fetrow, J.S.: Sulfenic acid-reactive compounds and their methods of synthesis. U.S. Patent 7,294,748, issued Nov. 13, 2007

Jönsson, T. J., Ellis, H. R., and Poole, L. B.: Cysteine reactivity and thiol-disulfide interchange pathways in AhpF and AhpC of the bacterial alkyl hydroperoxide reductase system. Biochemistry 46: 5709-5721 (2007). PDF of article.

Phalen, T. J., Weirather, K., Deming, P. B., Anathy, V., Howe, A. K., van der Vliet, A., Jönsson, T. J., Poole, L. B., and Heintz, N. H.: Oxidation state governs structural transitions in peroxiredoxin ii that correlate with cell cycle arrest and recovery. J Cell Biol 175:779-789 (2006). PDF of article.

Parsonage, D, Youngblood, D.S., Sarma. G.N., Wood, Z.A., Karplus, P.A., and Poole, L.B.: Analysis of the link between enzymatic activity and oligomeric state in AhpC, a bacterial peroxiredoxin. Biochemistry 44: 10583-10592 (2005). PDF of article.

Poole, L.B.: Bacterial defenses against oxidants: Mechanistic features of cysteine-based peroxidases and their flavoprotein reductases. Arch. Biochem. Biophys. 433: 240-254 (2005). PDF of article.

Poole, L.B., Karplus, P.A., and Claiborne, A. (2004) Protein sulfenic acids in redox signaling. Annu. Rev. Pharmacol. Toxicol. 44: 325-347. PDF of article.

Wood, Z.A., Poole, L.B., and Karplus, P.A. (2003) Peroxiredoxin evolution and the regulation of hydrogen peroxide signaling. Science 300: 650-653. PDF of article. PDF of supplementary material. Press Releases

Wood, Z.A., Schröder, E., Harris, J.R., and Poole, L.B. (2003) Structure, mechanism and regulation of peroxiredoxins. Trends Biochem. Sci. 28, 32-40. PDF of article.

Wood, Z.A., Poole, L.B., Hantgan, R.R., and Karplus, P.A. (2002) Dimers to doughnuts: Redox-sensitive oligomerization of 2-cysteine peroxiredoxins. Biochemistry 41, 5493-5504. PDF of article.

Ritz, D., Lim, J., Reynolds, C.M., Poole, L.B., and Beckwith, J. (2001) Conversion of a peroxiredoxin into a disulfide reductase by a triplet repeat expansion. Science 294, 158-160. PDF of article

Other Publications