Transgenic Mouse Core Facility
Mission
The Transgenic Mouse Core Facility provides investigators at Wake Forest University Health Sciences (WFUHS) access to state-of-the-art technologies for the generation of genetically-engineered mouse lines.
Services
Consultation with the Core Director regarding the strategy for production and testing of the transgenic and gene targeting constructs
Pronuclear microinjection of DNA into fertilized mouse embryos for the production of transgenic mouse lines
Gene targeting in mouse embryonic stem (ES) cells
Injection of gene-targeted ES cells into mouse blastocysts to produce chimeric mice for the creation of Knock-out/Knock-in mouse lines
Embryo rederivation for eradication of horizontally-transmitted, post-implantation, and vertically transmitted pathogens
Mouse genotyping from tail clips, using polymerase chain reactionæ(PCR) procedures
Details
About Transgenic Mice
About Knock-out/Knock-in Mice
About Embryo Rederivation
About Mouse Genotyping Service
Procedure Request Forms
Consultation with the Core Director regarding the strategy for production and testing of the transgenic and gene targeting constructsPronuclear microinjection of DNA into fertilized mouse embryos for the production of transgenic mouse linesGene targeting in mouse embryonic stem (ES) cellsInjection of gene-targeted ES cells into mouse blastocysts to produce chimeric mice for the creation of Knock-out/Knock-in mouse linesEmbryo rederivation for eradication of horizontally-transmitted, post-implantation, and vertically transmitted pathogens Mouse genotyping from tail clips, using polymerase chain reaction (PCR) procedures
Staff
Liqing Yu, MD PHD
Director, Transgenic Mouse Core Facility
Lihong Shi, MD
Transgenic Mouse Specialist
Feng Guo, MD
Laboratory Manager
Additional Information:
These Cell Migration Consortium/Nature Publishing Group websites provide useful background or resource material for those unfamiliar with the procedures:
- Beginners with Transgenic or Knockout Mice, start here.
- For a guide to the basics of preparing Gene Targeting Constructs, go here.
Note:
If you cannot make your own gene targeting constructs, there are companies that will make gene targeting constructs for a fee. The Transgenic Mouse Core staff have not had direct experience with any of these companies, but would be happy to provide investigators with their names. Call Dr. Liqing Yu at 716-0920 for more information.
ES Cell Lines Available for Generating Some Knockout Mice
Some knockout mice can be made without the investigator having to generate the targeting constructs or the targeted mouse embryonic cells.æ Please check the following links to see if the gene of interest has been targeted in the mouse embryonic stem cells or even in mice.
The International Mouse Knockout Consortium:
National Institutes of Health Knockout Mouse Project (KOMP)
European Conditional Mouse Mutagenesis Program (EUCOMM)
North American Conditional Mouse Mutagenesis Project (NorCOMM)
Other Useful Weblinks:
International Gene Trap Consortium
The Mouse Genome Informatics
Mutant Mouse Regional Resource Centers
International Mouse Strain Resource (IMSR)
Texas Institute for Genomic Medicine (TIGM)
Call the Transgenic Mouse Core Facility's director, Dr. Liqing Yu, at 716-0920 for more information.
Internal Advisory Committee
Richard St. Clair, PhD
Chair Pathology - Lipid Science
Paul Dawson, PhD Internal Medicine - Gastroenterology, Pathology - Lipid Scienc
John Parks, PhD Pathology - Lipid Science
Larry Rudel, PhD Pathology - Lipid Scienc
Sara Jones, PhD Physiology/Pharmacology
