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Research Areas:
Kinetic Modeling of Redox Signaling Networks, Elucidation of PDGF Signaling Cascades in Cancer and Heart Disease, CO Signaling
Current Research:
The overall objectives of our research are: i] investigate the molecular mechanisms that control redox modulated signaling networks and, ii] quantify the effect of oxidation on the timing of signaling events. To understand these fundamental features of cell signaling, we apply novel proteomics methods based on kinetics and mass spectrometry. In this context, we are interested in two major research areas:
(1) Mapping phosphorylation events associated with oxidative and radiation
damage esponse; the effect of these events on cell cycle regulation.
(2) The effect of carbon monoxide on signaling networks. The potential of carbon
monoxide (CO) to act as enzyme activity modulator and regulator of
transcription has only recently been established. However, less is known
about the effect of CO on signaling pathways. To that end, we will apply mass
spectrometry based proteomics to uncover dynamic changes in specific
signaling pathways in response to increased levels of CO.
(3) Cell signaling networks modeling to achieve a better understanding of the
events leading to pathological changes in signaling networks.
(4) Clinical proteomics to identify potential targets for cancer therapies.
(5) Development of Milliseconds Timescale Kinetics Chips for Quantitative
Monitoring of Enzyme Catalysis by MS
  
Customized Nanokinetics Chip moves into spray position
Publications:
Thomas J. Jönsson, Allen W. Tsang, W. Todd Lowther, and Cristina M. Furdui Identification of intact protein thiosulfinate intermediate in the reduction of
cysteine sulfinic acid in peroxiredoxin by human sulfiredoxin J. Biol. Chem., Jun 2008
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