Research Interests: Study the effects of Nitric Oxide and Aggregation in biological systems, especially as it relates to diseases such as Sickle Cell Disease.. Current Research: Dr. Kim-Shapiro is a biophysicist who uses light (especially polarized light) and other techniques to Investigate the effects of nitric oxide and hydroxyurea on sickle cell hemoglobin using EPR and other spectroscopies. This work is conducted in collaboration with Bruce King of the Chemistry Department. Study the mechanism and kinetics of sickle cell hemoglobin polymer melting (unaggregation). Sickle Cells
Investigate the structure of the sickle cell hemoglobin fiber (aggregate). A sickle cell hemoglobin gel as observed through crossed polarizers 
Recent Publications: Grubina R, Huang Z, Shiva S, Joshi MS, Azarov I, Basu S, Ringwood LA, Jiang A, Hogg N, Kim-Shapiro DB, Gladwin MT. Concerted nitric oxide formation and release from the simultaneous reactions of nitrite with deoxy- and oxyhemoglobin. J Biol Chem. 2007 Apr 27;282(17):12916-27. Epub 2007 Feb 23. Huang KT, Huang Z, Kim-Shapiro DB. Nitric oxide red blood cell membrane permeability at high and low oxygen tension. Nitric Oxide. 2007 Mar;16(2):209-16. Epub 2006 Dec 5. Doctor A, Gaston B, Kim-Shapiro DB. Detecting physiologic fluctuations in the S-nitrosohemoglobin micropopulation: Triiodide versus 3C. Blood. 2006 Nov 1;108(9):3225-6; author reply 3226-7. Jeffers A, Gladwin MT, Kim-Shapiro DB. Computation of plasma hemoglobin nitric oxide scavenging in hemolytic anemias. Free Radic Biol Med. 2006 Nov 15;41(10):1557-65. Epub 2006 Aug 25. Gladwin MT, Raat NJ, Shiva S, Dezfulian C, Hogg N, Kim-Shapiro DB, Patel RP. Nitrite as a vascular endocrine nitric oxide reservoir that contributes to hypoxic signaling, cytoprotection, and vasodilation. Am J Physiol Heart Circ Physiol. 2006 Nov;291(5):H2026-35. Epub 2006 Jun 23. Review. Publications:
For a listing of additional publications, refer to PubMed, a service provided by the National Library of Medicine |