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Current Research:
More than 2,600 Americans die of cardiovascular disease each day, an average of 1 death every 33 seconds. After an acute myocardial infarction, reperfusion may be established as a means to salvage functional myocardial tissue. However, many times, even after reperfusion, the myocardium has often times suffered an extreme injury and cannot be regenerated. Cell therapy has been proposed as a means to promote the regeneration of injured heart muscle. We have established lines of broad spectrum multipotent stem cells derived from primitive fetal cells present in human and mouse amniotic fluid (AFS) cells. AFS cells offer several advantages: They are easy to isolate and grow (no feeder layers needed), are highly expansive including clonal growth and they can differentiate into all germ layers (see reference De Coppi et al. Nat Biotech 2007;25(1):100-6). Despite these obvious scientific and clinical advantages, the cardiac lineage in vitro and in vivo has not been fully investigated. The goals of my research are to investigate this novel source of stem cells and the cardiac lineage, and the potential for the regeneration of injured myocardium from this novel source of stem cells. In this study, we hypothesize that AFS cells can differentiate into cardiac muscle cells and be used for cardiac tissue regeneration
Honors/Awards:
2006 Wake Forest Institute for Regenerative Medicine Annual Retreat-Pre-Doctoral Poster Award for “MRI Tracking of Stem Cells”
2006 Invited by the NIH to attend the Annual NIH Graduate Student Research Festival: October 10-11, 2006
2006 Wake Forest University Alumni Student Travel Award
Wake Forest University Graduate School of Arts and Sciences
To attend the Scientific Sessions of the American Heart
Association, Chicago, IL, 2006
2005 Wake Forest University Alumni Student Travel Award
Wake Forest University Graduate School of Arts and Sciences
To attend the Keystone Symposia on Cardiac Regeneration
Santa Fe, NM, 2005
2003 Invited Senior Thesis in Biology
Eckerd College
Saint Petersburg, FL 33711
2003 American Association of University Women-Florence Siebert
Research Award
Eckerd College
Saint Petersburg, FL 33711
2001 Eckerd College Natural Sciences Summer Research Program
Howard Hughes Foundation
Eckerd College
Saint Petersburg, FL 33711
Publications:
POSTERS
Delo, DM, Olson, J., Atala, A. Soker, S; Zhu, JM; Mouse Cardiac MR Imaging of Human Amniotic Fluid Stem Cells for Longitudinal Cell Graft Monitoring; Wake Forest Graduate School Research Day 2008
Brown, C., Wang, X. Delo, DM, Tranh, T., Burcin, M. Cultivation and Manipulation of Human Embryonic Stem Cells; Novartis Institute for Biomedical Research Heart Failure Group Retreat; September, 2007
Delo, DM, Olson, J., Atala, A. Soker, S; Zhu, JM; Mouse Cardiac MR Imaging of Human Amniotic Fluid Stem Cells for Longitudinal Cell Graft Monitoring; International Society for Stem Cell Research, June 2007
Delo DM, Eberli, D, Atala A, Soker S; Engineering of Muscle Tissue from Aging Animals; International Society for Stem Cell Research, June 2007
Delo, DM; Shaffer, R; Groban, L; Callahan, M; Atala, A; Soker, S; Human Amniotic Fluid Derived Stem Cells for Cardiac Therapeutics; International Society for Stem Cell Research, June 2007
Delo, DM, Olson, J., Atala, A. Soker, S; Zhu, JM: High Resolution MRI Tracking of Stem Cells in the Mouse Heart; Wake Forest University Graduate Student Research Day, 2007
Delo, DM, Neshat-Vahid, S*; DeCoppi, P; Milanesi, A; Christ, G; Soker, S; Atala, A. Amniotic Fluid-derived Stem Cells for Cardiac Therapeutics, Scientific Sessions American Heart Association National Meeting, 2006
Delo, DM, Olson, J., Atala, A. Soker, S; Zhu, JM: High Resolution MRI Tracking of Stem Cells in the Mouse Heart; Wake Forest University Surgical Sciences Research Day, 2006
Delo, DM, Neshat-Vahid, S*; DeCoppi, P; Milanesi, A; Christ, G; Soker, S; Atala, A. Amniotic Fluid-derived Stem Cells for Cardiac Therapeutics, Wake Forest Surgical Sciences Research Day, 2006
Delo, DM, Neshat-Vahid, S*; DeCoppi, P; Milanesi, A; Christ, G; Soker, S; Atala, A. Amniotic Fluid-derived Stem Cells for Cardiac Therapeutics, International Society for Stem Cell Research Conference, June 2006.
Delo, DM, Neshat-Vahid, S*; DeCoppi, P; Milanesi, A; Christ, G; Soker, S; Atala, A. Amniotic Fluid-derived Stem Cells for Cardiac Therapeutics, Wake Forest University Graduate School Research Day, 2006
Delo, DM, Neshat-Vahid, S*; DeCoppi, P; Milanesi, A; Christ, G; Soker, S; Atala, A. Amniotic Fluid-derived Stem Cells for Cardiac Therapeutics, Keystone Symposia on Cardiac Regeneration and Disease, 2006
Delo DM, Koh C, Lanza R, Lee J, Yoo J, Soker S., Atala, A. Parthenogenesis-derived Multipotent Stem Cells for Tissue Engineering Applications, International Society for Stem Cell Research Conference, June 2005.
Delo DM, Eberli D, Atala A, Soker S. Engineering of Aging Muscle Tissue, Wake Forest University Graduate Student Research Day, 2005
Nomi M., Soker S., Delo DM, Eberli D., DeCoppi P., Yoo J, Atala A. Enhancement of Muscle Regeneration Using Vascular Endothelial Growth Factor(VEGF) and Endothelial Cells, Wake Forest Surgical Sciences Research Day, 2004
Nomi M., Soker S., Delo DM, Eberli D., DeCoppi P., Yoo J, Atala A. Enhancement of Muscle Regeneration Using Vascular Endothelial Growth Factor(VEGF) and Endothelial Cells, NCTEIG Meeting, 2004
Koh C.,Lanza R., Lee J., Chen K., Delo DM., Hipp J., Soker S., Yoo J., Atala A. Parthenogenesis: A Novel Source of Stem Cells for Reconstruction; NCTEIG Meeting, 2004
Lipscomb K, Delo DM, Nascone NM: Ectopic retinoic acid induces three-dimensional patterning defects in the digestive system. Society of Developmental Biology Conference, 2002
Delo DM, Kakoullis J, Schnabel C, Guida WC: Zinc Model Systems. American Chemical Society Conference, 2002
PRESENTATIONS
Delo DM, Amniotic Fluid Stem Cells for Regenerative Medicine Applications, October 2007, (Novartis Institute for Biomedical Research, Cambridge, MA)
Delo DM, Amniotic Fluid Derived Stem Cells for Regenerative Therapy of Chronic Heart Disease, June 2007, (International Society for Stem Cell Research Conference, Cairns, Australia)
Delo DM, Approaches to Muscle Regeneration, May 2007 (Duke University Medical School, Durham, NC)
Delo DM, A Novel Source of Stem Cells for Cardiac Regeneration, June 2006 (Wake Forest Institute for Regeneration Seminar Series, Winston-Salem, NC)
Delo DM, Engineering of Aging Skeletal Muscle Tissue, June 2005 (Regenerate 2005 Conference, Atlanta, GA)
Delo DM, Skeletal and Cardiac Muscle Regeneration; May 6, 2005 (Wake Forest Health Sciences, Winston-Salem, NC)
Delo DM, Muscle Tissue Engineering. April 23, 2004 (Wake Forest Health Sciences, Winston-Salem, NC)
Delo DM, “Protein Therapy”: Non-viral Delivery of Proteins to Mitochondria. November 21, 2003 (Wake Forest Health Sciences, Winston-Salem, NC)
Delo DM, High Throughput Screening by In Situ Hybridization of a Heart Specific cDNA Library: Senior Thesis Presentation: May 8, 2003 (Eckerd College, St. Petersburg, FL)
PUBLICATIONS
Delo DM., Wang Z., Shaffer R., Guan, X., Neshat, S., Groban,L., Callahan, M., Smith, T., D’ Agostino Jr., R. Atala, A., ,Soker, S. The Effects of Human Amniotic Fluid-Derived Stem Cell Transplantation on the Infarcted Heart . (manuscript in submission)
Delo, DM., Guan, X., Wang, Z., Groban, L., Callahan, M., Smith, T.,Payne, R.M., Sane, D., Atala, A., Soker, S. Unexpected Severe Calcification after Myocardial Infarction in Rats Is Not Caused by Amniotic Fluid Stem Cells. (manuscript in submission)
Delo, DM., Eberli, D. Williams, J. Andersson, K.E., Atala, A., Soker S., The Effects of Using Angiogenic Gene Modified Skeletal Muscle Cells to Compensate for Aging-induced Changes in Bioengineered Functional Muscle Tissue (in press, British Journal of Urology)
Delo, DM., Olson, J., Baptista, P., Zhu, JM., Soker, S. Non-Invasive Longitudinal Tracking of Human Amniotic Fluid Stem Cells in Mouse Heart (in press, Stem Cells and Development)
Delo, DM., Baptista, P., Soker, S., Atala, A. Amniotic Fluid Pluripotent Stem Cells; Cell Therapy 1st Edition, Book Chapter, 2008
Halum, SL., Naidu, M., Delo, DM, Atala, A.,Hingtgen, C. Injection of Autologous Muscle Stem Cells (Myoblasts) for the Treatment of Vocal Fold Paralysis; Laryngoscope, May; 117(5): 917-22
Delo, DM, Shaffer, R., Atala, A., Soker, S. Cell and Tissue Engineering-Based Therapies for Heart Disease; [DVD Monograph] Society of Cardiac Anesthesiologists, April 2007
Delo DM, DeCoppi, P., Bartsch, G., Atala, A (2006) Amniotic Fluid and Placental Stem Cells; Methods In Enzymology volume on Embryonic Stem Cells, 416: 426-438.
De Coppi, P., Delo DM, Farrugia, L., Udompanyanan, K., Yoo, JJ, Nomi, M., Atala A., and Soker S(2005) Angiogenic gene modified muscle cells for enhancement of tissue formation; Tissue Engineering, Jul-Aug;11(7-8):1034-44
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