Module A:  Cell Physiology/Biochemistry/Proteomics

• Characterization of cellular protein expression, signaling events, and function in cell culture models
• Development of primary cell cultures (including those derived from human donor tissue)
• Employment of molecular transfection/infection strategies if necessary
• This module has greatest potential to interact with clinical research project

Structural and phenotypic characterization of murine ASM cultures derived from C57BL/6 mice

Quantification of phospho- and total proteins using the Odyssey Imaging System

Phospho
p42/p44

Total
p42/p44

 
Merge

2-D gel analysis of differential protein expression in GFP or PKI-GFP – expressing human ASM cells stimulated 18 h with 1L-1b and TNF-a.

GFP

PKI-GFP